- Chula researchers developed a simple, accurate, and fast urine test kit for 
cervical cancer that's got the nod from the National Research Council of 
Thailand's 2021 Outstanding Inventions Award.  


Cervical cancer is the second most common cancer in Thai women age 30-60, after 
breast cancer, with 10,000 new cases per year, and claiming as many as 5,000 
lives annually. Though curable if detected early, many women are afraid of 
getting tested due to the cost, and their fear of the Pap Smear method. It is a 
sexually transmitted disease caused by the human papillomavirus (HPV), not 
preventable by condoms. And though there is a vaccine for HPV, it's not yet 
prevalent, nor does it provide 100% protection.

Today, the Department of Chemistry, Faculty of Science, Chulalongkorn 
University's research team, comprising Prof. Dr. Orawan Chailapakul, Dr. 
Prinjaporn Tee-ngam, Sarida Naorungroj, Dr. Somrak Petchcomchai, and Prof. Dr. 
Tirayut Vilaivan have invented the urine HPV paper-based DNA sensor testing 
kits for cervical cancer that would allow a simpler and pain-free procedure 
that can be done frequently.

The urine HPV paper-based DNA sensor testing kits are easier and much faster 
than the Pap Smear. It eliminates the need to insert a tool and hold the 
vaginal walls open.

"The kits test for HPV DNA using PNA (Peptide Nucleic Acid), a synthetic 
substance that imitates our DNA, and designed to target specific cancer-causing 
strains of the virus," said Prof. Dr. Orawan. 

"Urine sample is mixed in a solution, then dropped onto the paper sensor, so 
the color change can be observed. The normal color is bright red, and it gets 
lighter according to the amount of the virus detected. Urine has fewer DNA 
materials than tissues, so a smartphone colorimeter app amplifying the DNA 
level is used alongside to make color change more visible." Test results show 
the test kit's high accuracy with a sensitivity of 85%, specificity of 78%, and 
reproducibility rate of 100%."

The less-than-500-Baht HPV Testing Kits are not for home use and must be 
administered by health centers and community hospitals. 

To stay safe from cervical cancer, women should "always keep the vaginal area 
clean, and get regular checkups," Prof. Dr. Orawan concluded.

For more information, visit https://www.chula.ac.th/en/highlight/67741/

Media Contact:
Chulalongkorn University Communication Center
Email: Pataraporn.r@chula.ac.th

Research Team Contact:
Prof. Dr. Orawan Chailapakul
Email: orawon.c@chula.ac.th


SOURCE: Chulalongkorn University Communication Center

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   Caption: HPV Testing Kits - Chula’s Innovation for Women’s Health